Hands-on courses for the exploration of molecular environmental diversity

Sequence Similarity Networks

In the SSNetworks GitHub repository you will find all necessary materials for getting started with sequence similarity networks with (mostly) DNA sequences.

Phylogenetic analyses

The phylogeniesQuickStart GitHub repository is intended to be a practical guide for the reconstruction and specially the interpretation of phylogentic trees of (mostly) DNA sequences.

Scripts and pipelines for the exploration of molecular diversity

A collection of scripts for bioinformatics

In the random GitHub repository you will find a collection of a set of scripts to deal with common fasta files and phylogenetic tree manipulations from the command line.

Oligonucleotide design

The OligoN-design GitHub repository describes a pipeline for the high-throughput design of specific primers or probes for further molecular analyses, such as Fluorescence in situ Hybridization (FISH) or PCR amplification.

Wet-lab protocols for single-cell

DNA amplification

Here you can find protocols for single-cell ribosomal DNA extraction and amplification of Nassellaria and Spumellaria (Radiolaria).

Scanning electron and confocal microscopy imaging of silica skeletons

Here you can find a protocol for cleaning and staining of silica skeletons for confocal microscopy imaging (CM) and a protocol for cleaning and mounting silica skeleton for scanning electron microscopy imaging (SEM). In general this protocols will work for most skeleton that do not dissolve on water.

Silhouettes

16 silhouettes of Radiolaria

I have deposited in the PhyloPic database 16 silhouettes of Radiolaria to the public domain for further presentations, manuscripts and outreach purposes.
These silhouettes are rendered from real scanning electron microscopy images, and I'm slowly rendering and uploading more.